Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
Pharmacogenetics and Pharmacogenomics: Overview01:29

Pharmacogenetics and Pharmacogenomics: Overview

Pharmacogenetics and pharmacogenomics examine how genetic factors influence an individual's response to drugs. While pharmacogenetics focuses on the impact of specific genetic variants on drug effects, pharmacogenomics takes a broader approach, studying how genetic variation across populations contributes to differences in drug responses. These fields aim to explain why individuals may experience varying levels of efficacy or adverse reactions to the same medication.Variability in drug...
Drug Discovery: Overview01:26

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
Pharmacogenetics of Drug Metabolism: Overview01:27

Pharmacogenetics of Drug Metabolism: Overview

Genetic polymorphism in drug metabolism is crucial to the inter-individual variability observed in drug responses. Drug metabolism primarily involves the chemical modification of drugs and other xenobiotics to enhance their elimination by increasing their polarity. Two main classes of enzymes mediate this biotransformation process: Phase I enzymes, primarily cytochrome P450s, catalyze oxidation and reduction reactions, while other enzymes, such as esterases, mediate hydrolysis, and Phase II...
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu01:29

Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu

Genetic variations significantly influence drug response through pharmacokinetics, receptor interactions, and biologic milieu modifications. Pharmacokinetic alterations impact drug metabolism and clearance, affecting efficacy and toxicity. Variants in drug-metabolizing enzymes, such as CYP2C9 and CYP2C19, alter drug activation and elimination. For example, CYP2C9 loss-of-function variants require lower warfarin doses to prevent excessive bleeding, while CYP2C19 variants reduce clopidogrel...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromosome-scale genome assembly and characterization of Saccharomycopsis schoenii, a necrotrophic predatory yeast.

G3 (Bethesda, Md.)·2026
Same author

STARD10 regulates human pancreatic β cell differentiation and triglyceride metabolism.

Stem cell reports·2026
Same author

Virus Genome Sequences in the Blood of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients.

medRxiv : the preprint server for health sciences·2025
Same author

Correction: Programmed cell death and redox metabolism protect Chlamydomonas reinhardtii populations from the galactic cosmic environment on the Artemis-1 mission.

Scientific reports·2025
Same author

Impact of polymorphisms on gene expression and splicing in response to exercise and diet-induced weight loss in human skeletal muscle tissues.

Cell genomics·2025
Same author

Barcode sequencing: a robust, platform-agnostic method for massively parallel cell-based screens.

G3 (Bethesda, Md.)·2025

Related Experiment Video

Updated: Jun 8, 2026

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

Exploring gene function and drug action using chemogenomic dosage assays.

Elke Ericson1, Shawn Hoon, Robert P St Onge

  • 1Department of Pharmaceutical Sciences, University of Toronto, Toronto, Ontario, Canada.

Methods in Enzymology
|October 16, 2010
PubMed
Summary

This study introduces genome-wide assays like haploinsufficiency profiling (HIP), homozygous profiling (HOP), and multicopy suppression profiling (MSP) for understanding drug-gene interactions and gene function. These methods utilize pooled yeast strains to analyze drug sensitivity across the genome.

More Related Videos

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids
08:52

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

Published on: November 22, 2021

Related Experiment Videos

Last Updated: Jun 8, 2026

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids
08:52

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

Published on: November 22, 2021

Area of Science:

  • Genomics
  • Molecular Biology
  • Pharmacology

Background:

  • Understanding drug-gene interactions and small molecule mechanisms is crucial in drug discovery.
  • Genome-wide, cell-based assays offer a powerful approach to dissect complex biological systems.

Purpose of the Study:

  • To describe a series of genome-wide, cell-based assays for understanding drug-gene interactions and gene function.
  • To detail the application of haploinsufficiency profiling (HIP), homozygous profiling (HOP), and multicopy suppression profiling (MSP) in Saccharomyces cerevisiae.
  • To provide a framework for defining the mechanism of action of small molecules.

Main Methods:

  • Utilizing competitive growth of complex pooled yeast strain collections.
  • Employing high-density microarrays for high-throughput screening.
  • Applying genetic tools such as HIP, HOP, and MSP to analyze gene dosage effects.
  • Screening pooled strains in parallel to assess genome-wide drug sensitivity or resistance.

Main Results:

  • Demonstrated the utility of pooled screening for analyzing gene product contributions to drug response.
  • Generated a compendium of results from genome-wide screens.
  • Established a basis for network analysis of genetic interactions and drug mechanisms.

Conclusions:

  • The described assays provide a robust foundation for understanding gene function and drug mechanisms.
  • Pooled, parallel screening enables efficient genome-wide analysis of drug-gene interactions.
  • The compendium of results can facilitate large-scale network analysis of genetic and drug interactions.