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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Updated: Jun 8, 2026

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

A mouse model for human anal cancer.

Marie K Stelzer¹, Henry C Pitot, Amy Liem

¹McArdle Laboratory for Cancer Research, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.

Cancer Prevention Research (Philadelphia, Pa.)

|October 16, 2010

Summary

A new mouse model using HPV16 E6/E7 genes and DMBA chemical carcinogen successfully mimics human anal cancer. This breakthrough aids research into viral and cellular factors in anal carcinogenesis and therapeutic development.

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Last Updated: Jun 8, 2026

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

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Published on: July 6, 2017

Orthotopic Mouse Model of Colorectal Cancer

Orthotopic Mouse Model of Colorectal Cancer

Published on: December 4, 2007

Area of Science:

Oncology
Virology
Genetics

Background:

Human anal cancers are strongly linked to high-risk human papillomaviruses (HPV), particularly HPV16.
HPV16 is the predominant high-risk HPV type found in anal cancers, similar to other HPV-associated malignancies.

Purpose of the Study:

To develop and characterize a novel mouse model for human anal cancer.
To investigate the role of HPV16 E6 and E7 oncogenes in anal carcinogenesis using a transgenic mouse model.

Main Methods:

Generation of K14E6/K14E7 transgenic mice expressing HPV16 E6 and E7 genes in stratified squamous epithelia.
Topical application of dimethylbenz[a]anthracene (DMBA), a chemical carcinogen, to assess anal cancer susceptibility in transgenic and non-transgenic mice.
Histopathologic and biomarker analyses to characterize tumors and precancerous lesions.

Main Results:

HPV16 E6 and E7 genes were functionally expressed in the anal epithelia of transgenic mice.
DMBA treatment induced anal tumors in approximately 50% of HPV16 E6/E7 transgenic mice, while non-transgenic mice remained tumor-free.
The induced mouse anal cancers exhibited histopathologic and biomarker profiles similar to human HPV-positive anal precancers.

Conclusions:

The developed mouse model accurately recapitulates key features of human anal cancer.
This model provides a valuable platform for studying the interplay of viral and cellular factors in anal carcinogenesis.
The model is expected to facilitate the evaluation of novel therapeutic strategies for anal cancer prevention and treatment.