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Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Published on: December 21, 2016
Ambient particulate matter on DNA damage in HepG2 cells
Liping Jiang1, Hong Dai, Qinghua Sun
1China-Japanese Joint Institute for Medical and Pharmaceutical Science, Dalian Medical University, West Segment of South Lvshun Road, Dalian, Liaoning, China.
Toxicology and Industrial Health
|October 16, 2010
Summary
Particulate matter (PM10) in Dalian, China, causes DNA damage in liver cells, varying by season and location. This damage is linked to oxidative stress, 8-hydroxydeoxyguanosine, and NF-κB activation.
Area of Science:
- Environmental Health
- Molecular Toxicology
- Genetics
Background:
- Ambient particulate matter (PM) is linked to respiratory, cardiovascular, and lung diseases.
- Thoracic particles (PM10) contain extractable organic matter (EOM) that may induce cellular damage.
Purpose of the Study:
- To investigate seasonal and locational variations in DNA damage induced by PM10 EOM.
- To elucidate the mechanisms of DNA damage in human hepatoma G2 (HepG2) cells.
Main Methods:
- Single cell gel electrophoresis (SCGE) assay for DNA strand breaks.
- DCFH-DA assay for reactive oxygen species (ROS) production.
- Western blotting for NF-κB p65 protein accumulation.
Main Results:
- PM10 EOM induced significant DNA damage (tail DNA percentage) in HepG2 cells in a dose-dependent manner.
- DNA damage varied significantly between summer and winter seasons and among sampling sites.
- Increased ROS, 8-hydroxydeoxyguanosine (8-OHdG), and NF-κB p65 protein levels were observed.
Conclusions:
- PM10 EOM causes DNA strand breaks in HepG2 cells, with variations influenced by season and location.
- Mechanisms involve oxidative stress (ROS), 8-OHdG formation, and NF-κB p65 activation.
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