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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Interferometric Synthetic Aperture Microscopy: Computed Imaging for Scanned Coherent Microscopy.

Brynmor J Davis1, Daniel L Marks, Tyler S Ralston

  • 1The Beckman Institute for Advanced Science and Technology and The Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, IL 61801, USA.

Sensors (Basel, Switzerland)
|October 16, 2010
PubMed
Summary
This summary is machine-generated.

Interferometric Synthetic Aperture Microscopy (ISAM) enables 3D microscopy image formation without scanning focus. This computed imaging technique removes blur, offering advantages over traditional methods like optical coherence tomography for volumetric imaging.

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Area of Science:

  • Microscopy
  • Optical Imaging
  • Image Processing

Background:

  • Computed imaging techniques significantly enhance 3D microscopy.
  • Interferometric Synthetic Aperture Microscopy (ISAM) addresses out-of-focus blur in coherent microscopy.
  • Optical coherence tomography (OCT) requires focal scanning for volumetric data.

Purpose of the Study:

  • To describe Interferometric Synthetic Aperture Microscopy (ISAM).
  • To explore the relationship between ISAM and Synthetic Aperture Radar (SAR).
  • To compare ISAM with OCT and SAR within a common mathematical framework.

Main Methods:

  • Utilizes computed imaging techniques for 3D image formation.
  • Employs signal processing similar to seismology's Fourier migration and SAR's Fourier reconstruction.
  • Reconstructs volumetric images from data acquired at a single focal depth.

Main Results:

  • ISAM effectively removes out-of-focus blur in broadband, coherent microscopy.
  • Eliminates the need for scanning focal depth, unlike OCT.
  • Demonstrates mathematical similarities between ISAM and strip-map SAR systems.

Conclusions:

  • ISAM offers an advanced approach to 3D microscopy image formation.
  • The principles of ISAM are closely related to SAR imaging techniques.
  • This review provides insights for researchers familiar with SAR.