Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Compact Hydraulic Head Auto-Regulating Module (CHARM) for long-term constant gravity-driven flow microfluidics.

Microsystems & nanoengineering·2025
Same author

Inertial Microfluidics Enables Functional Analysis of Neutrophils Isolated from Ultralow Blood Volume Samples.

Analytical chemistry·2025
Same author

Learning and diSentangling patient static information from time-series Electronic hEalth Records (STEER).

PLOS digital health·2024
Same author

Optical tweezing of microparticles and cells using silicon-photonics-based optical phased arrays.

Nature communications·2024
Same author

Rapid, low-cost fabrication of electronic microfluidics via inkjet-printing and xurography (MINX).

Biosensors & bioelectronics·2023
Same author

A Multidatabase ExTRaction PipEline (METRE) for facile cross validation in critical care research.

Journal of biomedical informatics·2023

Related Experiment Video

Updated: Jun 8, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Cell-based sensors for quantifying the physiological impact of microsystems.

Salil P Desai1, Joel Voldman

  • 1Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Integrative Biology : Quantitative Biosciences From Nano to Macro
|October 16, 2010
PubMed
Summary

This study introduces a novel cell-based sensor and microfluidic platform for large-scale physiological screening of microsystems. It reveals how electrical parameters like voltage and frequency impact cellular stress responses.

More Related Videos

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

Related Experiment Videos

Last Updated: Jun 8, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

Area of Science:

  • Biotechnology
  • Cellular Physiology
  • Microfluidics

Background:

  • Microsystems are essential for cell manipulation, patterning, and sorting.
  • Understanding microsystems' impact on cellular physiology is crucial for their adoption.

Purpose of the Study:

  • To develop and integrate a cell-based sensor with a microfluidic platform for large-scale physiological screening.
  • To investigate the impact of electrical parameters on cellular physiology within microsystems.

Main Methods:

  • Development of a cell-based sensor reporting stress-mediated transcription (Heat Shock Factor 1-GFP).
  • Quantitative characterization of the sensor using automated imaging.
  • Integration with a microfabricated electrical screening platform for multiplexed screens.

Main Results:

  • Physiological stress increases with voltage due to Joule heating.
  • Lower frequencies (<500 kHz) induce more stress than higher frequencies (>1 MHz) due to reactive species generation.
  • Combined voltage and frequency sweeps generate complex physiological state maps.

Conclusions:

  • The integrated system enables the first large-scale physiological screens of microsystem impacts on cells.
  • Electrical field strength, frequency, and duration significantly influence cellular physiological responses.
  • This platform facilitates a deeper understanding of cell-microsystem interactions.