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A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Published on: April 24, 2020
Blood plasma handling for protein analysis
Christer Ericsson1, Monica Nistér
1Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden. christer.ericsson@ki.se
Methods in Molecular Biology (Clifton, N.J.)
|October 16, 2010
Summary
Standard blood handling procedures ensure reliable protein analysis. These established methods are recommended for new protein biomarker discovery, ensuring consistent sample quality for research and clinical applications.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Molecular Biology
Background:
- Established blood handling protocols ensure consistent protein analytic results in clinical practice.
- Leveraging this experience is logical for developing new protein biomarker discovery methods.
- Standard blood sample handling precautions are applicable to new biomarker discovery.
Purpose of the Study:
- To outline a blood sample handling protocol suitable for new protein biomarker discovery.
- To emphasize the importance of consistent sample handling for reliable analytical outcomes.
Main Methods:
- Collecting platelet-poor EDTA plasma at room temperature or 4°C within 90 minutes of venipuncture.
- Handling and screening samples to eliminate hemolysis.
- Isolating DNA from the buffy coat after plasma sedimentation.
Main Results:
- The described protocol yields consistent results for protein analysis.
- The protocol is adaptable for new biomarker discovery, ensuring sample integrity.
- Hemolysis is effectively screened and eliminated.
Conclusions:
- Existing blood handling routines provide a strong foundation for new protein biomarker discovery.
- Adherence to standardized protocols ensures the quality and reliability of biological samples for analysis.
- The described method facilitates the discovery of novel protein biomarkers through consistent sample preparation.
