Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipophilic anilino squaraine dye as a novel superluminescent probe with efficient two-photon transition and high photostability.

Physical chemistry chemical physics : PCCP·2026
Same author

Nonlinear optical spectroscopy of new squaraine derivatives with high potential for multidisciplinary applications.

Physical chemistry chemical physics : PCCP·2025
Same author

Neutral rhodol-based dyes expressing localization in mitochondria.

Organic & biomolecular chemistry·2024
Same author

Review of Recent Computational Research on the Adsorption of PFASs with a Variety of Substrates.

International journal of molecular sciences·2024
Same author

Novel BODIPY-Based Photobase Generators for Photoinduced Polymerization.

ACS applied materials & interfaces·2023
Same author

Nature of Linear Spectral Properties and Fast Relaxations in the Excited States and Two-Photon Absorption Efficiency of 3-Thiazolyl and 3-Phenyltiazolyl Coumarin Derivatives.

ACS omega·2023
Same journal

Donor-Acceptor Separation Augments Temperature Dependence of Kinetic Isotope Effects in NADH Model Hydride Transfer Reactions: Mimicking Enzyme versus Mutant Dynamics.

The journal of physical chemistry. B·2026
Same journal

Disordered Worm-Like Clusters in a Hexagonal Mesophase Former: Simulation and Thermodynamic Description.

The journal of physical chemistry. B·2026
Same journal

Comparative Biophysical Analysis of Healthy and Inflamed Intestinal Membrane Models Using Langmuir Monolayers.

The journal of physical chemistry. B·2026
Same journal

Phosphoserine Charge State Drives Ion Condensation and Spatial Polyamine Presentation in Multirepeat Silaffin.

The journal of physical chemistry. B·2026
Same journal

pH-Dependent Conformational Transition of the Glutamate-GABA Antiporter GadC Revealed by <sup>19</sup>F NMR.

The journal of physical chemistry. B·2026
Same journal

Hydrogen-Bond Network in Equimolar <i>N</i>-Methylacetamide-Water: Integrated Neutron Scattering, Molecular Dynamics, DFT-NBO-AIM, and Machine Learning Analysis.

The journal of physical chemistry. B·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

New two-photon-absorbing probe with efficient superfluorescent properties.

Kevin D Belfield1, Carolina D Andrade, Ciceron O Yanez

  • 1Department of Chemistry, University of Central Florida, P.O. Box 162366, Orlando, Florida 32816-2366, USA.

The Journal of Physical Chemistry. B
|October 19, 2010
PubMed
Summary
This summary is machine-generated.

This study details a novel fluorescent probe (1) with excellent photophysical properties, including high quantum yield and stability. Its potential for bioimaging applications was demonstrated through cell imaging and amplified stimulated emission, suggesting use in advanced fluorescent labels.

More Related Videos

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Related Experiment Videos

Last Updated: Jun 8, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Area of Science:

  • Organic Chemistry
  • Photophysics
  • Biomedical Imaging

Background:

  • Fluorescent probes are essential tools in biological research and diagnostics.
  • Developing probes with enhanced photophysical properties, such as high quantum yield and photostability, is crucial for sensitive imaging.
  • Two-photon absorption (2PA) offers advantages for deep-tissue imaging due to reduced phototoxicity and improved spatial resolution.

Purpose of the Study:

  • To synthesize and characterize a novel organic molecule (1) for potential use as a fluorescent probe.
  • To investigate the linear photophysical, photochemical, two-photon absorption (2PA), and superfluorescence properties of compound 1.
  • To evaluate the suitability of probe 1 for bioimaging applications, including cell imaging and amplified stimulated emission.

Main Methods:

  • Synthesis of the dibenzothiazole-thiophene-fluorene derivative (1).
  • Steady-state absorption, fluorescence, and excitation anisotropy spectroscopy in various solvents.
  • Two-photon absorption (2PA) spectra determination using femtosecond pulsed laser excitation.
  • One- and two-photon fluorescence microscopy of HCT-116 cells.
  • Amplification of stimulated emission measurements.

Main Results:

  • Compound 1 exhibited high fluorescence quantum yield (~0.7-0.8) and excellent photochemical stability.
  • A significant two-photon absorption band was observed between 680-720 nm with cross-sections up to ~900 GM.
  • Probe 1 demonstrated successful one- and two-photon fluorescence imaging of HCT-116 cells.
  • Amplified stimulated emission was achieved in organic solvents and thin films, indicating potential for enhanced spectral brightness.

Conclusions:

  • The synthesized compound 1 possesses favorable photophysical and photochemical properties for fluorescent probe applications.
  • Its demonstrated performance in cell imaging and potential for amplified stimulated emission highlight its utility in advanced bioimaging and fluorescent labeling.
  • Compound 1 represents a promising candidate for developing next-generation fluorescent probes with superior spectral brightness and imaging capabilities.