Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity
Natalia N Tamm1, Alexander G Semenov, Karina R Seferian
1HyTest LTD, Intelligate, 6th floor, Joukahaisenkatu 6, 20520 Turku, Finland.
Objectives:
To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays.
Design And Methods:
Plasma samples (n=94) from HF patients were analyzed by the novel-type "Single Epitope Sandwich" (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli).
Results:
The SES assay measured 1.2- to 7.2-fold (2.1±0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference.
Conclusions:
The SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms.


