Related Experiment Video

Updated: Jun 8, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
08:31

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Published on: October 15, 2013

Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei

Guang Wen Sun1, Yunn-Hwen Gan

  • 1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8, Medical Drive, Singapore 117597.

Trends in Microbiology
|October 19, 2010
PubMed

Insights

Burkholderia pseudomallei, a versatile pathogen causing melioidosis, utilizes three type III secretion systems (T3SSs). Researchers modeled the T3SS3 apparatus to understand its role in disease.

Area of Science:

  • Microbiology
  • Pathogen Research
  • Infectious Diseases

Background:

  • Burkholderia pseudomallei is a versatile pathogen responsible for melioidosis.
  • Melioidosis is a significant human infectious disease with diverse clinical presentations.
  • The pathogen possesses a large genome and multiple virulence factors, including three type III secretion systems (T3SSs).

Purpose of the Study:

  • To elucidate the regulatory network of the T3SS3 in Burkholderia pseudomallei.
  • To characterize T3SS3 proteins.
  • To construct a model of the T3SS3 apparatus and assess its role in pathogenesis.

Main Methods:

  • Regulatory network analysis of T3SS3.
  • Characterization of T3SS3 proteins.
  • Construction of a T3SS3 apparatus model.

Main Results:

  • The regulatory network of T3SS3 was elucidated.
  • Several T3SS3 proteins were characterized.
  • A model of the B. pseudomallei T3SS3 apparatus was constructed.

Conclusions:

  • Understanding the T3SS3 apparatus and its regulatory network is crucial for evaluating its role in melioidosis pathogenesis.
  • Further research into T3SS3 mechanisms can provide insights into B. pseudomallei virulence.

More Related Videos

A Visual Assay to Monitor T6SS-mediated Bacterial Competition08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles09:55

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Published on: August 12, 2021

Related Concept Videos

Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
Bacterial Phylum Verrucomicrobiota01:26

Bacterial Phylum Verrucomicrobiota

The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
Determinants of Bacterial Pathogenicity and Virulence01:20

Determinants of Bacterial Pathogenicity and Virulence

Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...