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Ibandronate affects bone growth and mineralization in rats with normal and reduced renal function
Dagmar-Christiane Fischer1, Claudia Jensen, Anja Rahn
1Department of Pediatrics, University Children's Hospital Rostock, Ernst-Heydemann-Str. 8, 18057, Rostock, Germany. dagmar-christiane.fischer@med.uni-rostock.de
Pediatric Nephrology (Berlin, Germany)
|October 19, 2010
Summary
Ibandronate, a bisphosphonate, was found to impede tibial growth and bone mineralization in young rats. This effect was observed in both healthy rats and those with chronic renal failure (CRF).
Area of Science:
- Pharmacology
- Bone Biology
- Nephrology
Background:
- Bisphosphonates are known to reduce bone turnover.
- Their impact on endochondral bone formation, particularly in chronic renal failure (CRF), remains under-investigated.
- Ectopic calcification in experimental uremia can be attenuated by bisphosphonates.
Purpose of the Study:
- To investigate the effects of ibandronate on endochondral bone formation in rats with normal and reduced renal function.
- To determine if ibandronate impacts tibial growth and mineralization in the context of chronic renal failure.
Main Methods:
- Male Sprague-Dawley rats were administered weekly subcutaneous injections of vehicle or ibandronate (1.25 μg/kg) for 10 weeks.
- Rats were either sham-operated or 5/6 nephrectomized to induce chronic renal failure (CRF).
- Tibiae were analyzed after in vivo double labeling with tetracycline and demeclocycline.
Main Results:
- Ibandronate reduced body length gain and tibial growth rate in sham-operated rats but not in CRF rats.
- The proliferative zone of the epiphyseal growth plate was reduced by ibandronate in control rats and tended to be reduced in CRF rats.
- Mineral apposition rates were significantly reduced in ibandronate-treated rats, with a similar trend observed in CRF rats.
Conclusions:
- Ibandronate interferes with tibial growth and bone mineralization in young rats.
- The drug's impact on growth and mineralization may differ between rats with normal and reduced renal function.
- Further research is needed to fully elucidate the effects of bisphosphonates on bone formation in CRF.
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