Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Human Virome

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The alleviation of oxidative damage in celiac disease by black soybean peptides and their mechanism.

Journal of the science of food and agriculture·2026

Same author

Antimicrobial Mechanism of Temporin L Peptide Against Bacillus cereus from Fermented Sausage.

Probiotics and antimicrobial proteins·2026

Same author

Frequent exposure to biologics is associated with small intestinal bacterial overgrowth in patients with Crohn's disease: a retrospective case-control study.

PeerJ·2026

Same author

Clinical value of repeat kidney biopsy in pediatric IgA vasculitis nephritis.

Pediatric nephrology (Berlin, Germany)·2026

Same author

Processing-dependent hepatic risk and benefit of Polygonum Multiflorum Radix: Integrated chemical profiling, in vivo validation and candidate quality control markers.

Journal of pharmaceutical and biomedical analysis·2026

Same author

Intratumor <i>Lactobacillus</i> drives ferroptosis resistance via D-lactate-STAT3 K631 lactylation in esophageal squamous cell carcinoma.

Gut microbes·2026

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Marek's disease virus-encoded microRNAs: genomics, expression and function.

Jun Luo¹, Man Teng, JianMing Fan

¹Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.

Science China. Life Sciences

|October 19, 2010

Summary

Marek's disease virus (MDV) encodes microRNAs (miRNAs) involved in tumor formation. This review covers MDV miRNA discovery, function, and future research directions for understanding viral oncogenesis.

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Related Experiment Videos

Last Updated: Jun 8, 2026

MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Area of Science:

Virology
Molecular Biology
Oncology

Background:

MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression post-transcriptionally.
Viral genomes, particularly herpesviruses, encode numerous miRNAs.
Marek's disease virus (MDV) is an oncogenic alphaherpesvirus linked to T-lymphocyte transformation and tumor induction.

Purpose of the Study:

To review the discovery and identification of MDV-encoded miRNAs.
To summarize the genomics, expression profiles, and known functions of these viral miRNAs.
To discuss future research prospects and techniques for investigating MDV miRNA roles.

Main Methods:

Literature review of studies on MDV-encoded miRNAs.
Analysis of genomic data related to miRNA identification.
Compilation of expression profile and functional data from existing research.

Main Results:

MDV encodes a significant number of miRNAs with diverse regulatory roles.
These viral miRNAs are implicated in MDV's lytic replication, latency, and oncogenic potential.
Marek's disease serves as a valuable model for studying viral tumorigenesis.

Conclusions:

MDV-derived miRNAs are crucial players in the virus's life cycle and oncogenesis.
Further investigation into MDV miRNA functions is essential for understanding Marek's disease.
Advanced techniques will enhance the study of these viral small RNAs.