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Updated: Jun 8, 2026
04:59
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
Published on: April 12, 2019
Measurement of ALA synthase activity
P R Sinclair1, N Gorman, N W Cornell
1VA Medical Center, White River Junction, Vermont, USA.
Current Protocols in Toxicology
|October 19, 2010
Abstract:
In most cells, δ-aminolevulinate (ALA) synthase is the rate-limiting enzyme in heme synthesis. It is inducible by drugs and toxins and is feedback regulated by heme. This unit describes a radiometric assay using [¹⁴C]succinate as a substrate and a colorimetric assay based on the conversion of ALA to a pyrrole.
