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Differences between in vitro and in vivo obtained schistosomules
A L Melo1, C R Machado, L H Pereira
1Departamento de Parasitologia, Universidade Federal de Minas Gerais, Brasil.
Revista Do Instituto De Medicina Tropical De Sao Paulo
|March 1, 1990
Summary
Cell adhesion to Schistosoma mansoni larvae decreases as they develop into schistosomules. In vitro schistosomules showed cell adhesion for up to 24 hours, unlike in vivo schistosomules.
Area of Science:
- Parasitology
- Immunology
- Cell Biology
Background:
- Schistosoma mansoni cercariae transform into schistosomules after infecting a host.
- Cell adhesion to parasites is a key immune response.
- Differences between in vivo and in vitro derived schistosomules are not fully understood.
Purpose of the Study:
- To investigate the differences in cell adhesion properties between in vivo and in vitro derived Schistosoma mansoni schistosomules.
- To determine the time course of molecular changes related to cell adhesion during in vitro culture.
Main Methods:
- Injection of Schistosoma mansoni cercariae into the peritoneal cavity of mice.
- Isolation and in vitro culture of schistosomules.
- Assessment of cell adhesion to schistosomules at different time points (4 and 24 hours in vitro).
Main Results:
- In vivo derived schistosomules showed minimal cell adhesion upon reinfection.
- In vitro derived schistosomules exhibited significant cell adhesion at 4 and 24 hours.
- Complete removal of adhesion-mediating molecules in vitro appears to require more than 24 hours.
Conclusions:
- Schistosomule transformation in vitro does not fully replicate the in vivo immune evasion mechanisms regarding cell adhesion.
- In vitro culture conditions affect the expression or accessibility of surface molecules involved in host cell interactions.
- Further investigation is needed to elucidate the precise molecules and kinetics of their removal during in vitro culture.