Detection of biofilm formation among the isolates of staphylococcus epidermidis by modified microtiter- plate test

The present study was done to detect biofilm formation among the strains of Staphylococcus epidermidis isolated from different clinical specimens and healthy controls in the department of Microbiology, Mymensingh Medical College between July 2007 to June 2008. A total of 62 Staphylococcus epidermidis isolates were identified by standard microbiological technique. Among them 32 S. epidermidis were isolated from cases and 30 were from healthy controls. Antimicrobial susceptibility of the isolates was carried out by disk diffusion method as per recommendation of Clinical and Laboratory Standard Institute 2007. Biofilm production was detected by Modified microtiter-plate test. Out of 32 clinical isolates of S. epidermidis, 13 strains found to produce biofilm. On the other hand out of 30 S. epidermidis from healthy controls, only 5 strains produced biofilm. So detection rate of biofilm production of S. epidermidis by Modified microtiter plate test in clinical isolates was significantly higher than that of control group.