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Related Experiment Video

Updated: Jun 7, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Multicolor fluorescence enhancement from a photonics crystal surface.

A Pokhriyal, M Lu, C S Huang

Applied Physics Letters

|October 20, 2010

Summary

This study demonstrates a novel photonic crystal substrate that significantly enhances fluorescent signals for multiple dyes. This plastic device offers a 32x increase in signal intensity, improving biological assay sensitivity.

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Optics and Photonics
Materials Science
Biotechnology

Background:

Photonic crystals offer unique light-matter interaction properties.
Enhancing fluorescence signals is crucial for sensitive biological assays.
Existing methods for fluorescence enhancement have limitations in broad applicability.

Purpose of the Study:

To demonstrate a photonic crystal substrate for resonant enhancement of multiple fluorophores.
To investigate the substrate's performance with different laser excitation wavelengths and dyes.
To assess the potential for amplifying fluorescent signals in biological applications.

Main Methods:

Fabrication of a plastic photonic crystal substrate using nanoreplica molding over a ~3x5 in. surface area.
Utilizing two distinct resonant modes for electric field enhancement of cyanine-5 (λ=632.8 nm) and cyanine-3 (λ=532 nm) dyes.
Optimizing resonant coupling via distinct incident angles for each excitation wavelength.

Main Results:

Achieved a 32x increase in fluorescent signal intensity for cyanine-5 conjugated streptavidin.
Observed a 25x increase in fluorescent signal intensity for cyanine-3 conjugated streptavidin compared to glass.
Demonstrated amplification of fluorescent dyes with excitation wavelengths between 532 nm and 633 nm.

Conclusions:

The developed photonic crystal substrate effectively enhances fluorescence signals for multiple dyes.
This technology provides a significant improvement in sensitivity for biological assays.
The substrate is suitable for applications requiring multiplexed fluorescent detection, such as gene expression microarrays.