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Updated: Jun 7, 2026
08:32
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Inducible nitric oxide synthase expression.
1Kenneth S. Warren Laboratories, Tarrytown, New York, USA.
Current Protocols in Toxicology
|October 20, 2010
Summary
This study details three methods to measure inducible nitric oxide synthase (iNOS) expression in mouse macrophage cells. Lipopolysaccharide (LPS) stimulation was used to induce iNOS expression for analysis.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Inducible nitric oxide synthase (iNOS) plays a critical role in immune responses within macrophages.
- Understanding iNOS expression is crucial for studying inflammatory processes and macrophage function.
Purpose of the Study:
- To present three distinct protocols for quantifying iNOS expression in RAW 264.7 macrophage-like cells.
- To establish methods for measuring iNOS at the end product, protein, and mRNA levels.
Main Methods:
- Utilized RAW 264.7 cells, a common macrophage cell line.
- Stimulated iNOS expression using lipopolysaccharide (LPS), a bacterial product.
- Employed three complementary assays to measure iNOS: end product, protein level, and mRNA expression.
Main Results:
- Successfully demonstrated protocols for measuring iNOS expression in response to LPS stimulation.
- Validated methods for assessing iNOS at multiple molecular levels within the same cell type.
Conclusions:
- The presented protocols provide a comprehensive toolkit for researchers investigating iNOS in macrophage models.
- These methods facilitate a thorough analysis of iNOS regulation and function in cellular immunity.
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