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Related Experiment Video

Updated: Jun 7, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Inducible nitric oxide synthase expression.

¹Kenneth S. Warren Laboratories, Tarrytown, New York, USA.

Current Protocols in Toxicology

|October 20, 2010

Summary

This summary is machine-generated.

This study details three methods to measure inducible nitric oxide synthase (iNOS) expression in mouse macrophage cells. Lipopolysaccharide (LPS) stimulation was used to induce iNOS expression for analysis.

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Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Inducible nitric oxide synthase (iNOS) plays a critical role in immune responses within macrophages.
Understanding iNOS expression is crucial for studying inflammatory processes and macrophage function.

Purpose of the Study:

To present three distinct protocols for quantifying iNOS expression in RAW 264.7 macrophage-like cells.
To establish methods for measuring iNOS at the end product, protein, and mRNA levels.

Main Methods:

Utilized RAW 264.7 cells, a common macrophage cell line.
Stimulated iNOS expression using lipopolysaccharide (LPS), a bacterial product.
Employed three complementary assays to measure iNOS: end product, protein level, and mRNA expression.

Main Results:

Successfully demonstrated protocols for measuring iNOS expression in response to LPS stimulation.
Validated methods for assessing iNOS at multiple molecular levels within the same cell type.

Conclusions:

The presented protocols provide a comprehensive toolkit for researchers investigating iNOS in macrophage models.
These methods facilitate a thorough analysis of iNOS regulation and function in cellular immunity.