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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
Rapid isolation of intact chloroplasts from spinach leaves
David Joly1, Robert Carpentier
1Département de chimie biologie (GRBV), Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2010
Abstract:
In this chapter, a rapid method to isolate intact chloroplasts from spinach leaves is described. Intact chloroplasts are isolated using two short centrifugation steps and avoiding the use of percoll gradient. Intactness of chloroplast is evaluated by the inability of potassium ferricyanide to enter inside the chloroplasts and to act as an electron acceptor for photosystem II.
