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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Related Experiment Video

Updated: Jun 7, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
11:06

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Characterization of branched polysaccharides using multiple-detection size separation techniques.

Francisco Vilaplana1, Robert G Gilbert

  • 1The University of Queensland, Centre for Nutrition and Food Sciences (CNAFS) and School of Land, Crop and Food Sciences (LCAFS), Brisbane Queensland, Australia.

Journal of Separation Science
|October 21, 2010
PubMed
Summary
This summary is machine-generated.

Understanding branched polysaccharide structure is key to their function. This review highlights methods for analyzing branching and size distributions, crucial for applications in nutrition, health, and industry.

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Last Updated: Jun 7, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
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Published on: April 11, 2016

Area of Science:

  • Carbohydrate Chemistry
  • Polymer Science
  • Biophysical Chemistry

Background:

  • Branched polysaccharides exhibit complex hierarchical structures, influencing their biological and industrial applications.
  • Understanding the structure-function relationship is vital for optimizing polysaccharide use in diverse fields.
  • Current analytical methods face challenges in accurately characterizing complex branching and size distributions.

Purpose of the Study:

  • To review current state-of-the-art techniques for analyzing branched polysaccharide structure.
  • To focus on size-separation technologies coupled with sensitive detectors for branch chain and size distribution analysis.
  • To critically evaluate the limitations and challenges of existing analytical methodologies.

Main Methods:

  • Size-exclusion chromatography (SEC) coupled with multi-detector systems.
  • Analysis of number-, mass-, and molecular-weight-sensitive detection data.
  • Critical appraisal of established and emerging analytical protocols.

Main Results:

  • Size-separation techniques coupled with sensitive detectors offer powerful tools for characterizing branched polysaccharides.
  • Reliable determination of branch chain and size distributions is achievable with optimized methodologies.
  • Current technologies present specific challenges that require careful consideration and potential improvement.

Conclusions:

  • Accurate structural characterization of branched polysaccharides is essential for unlocking their full potential.
  • Advancements in size-separation and detection technologies are crucial for addressing analytical challenges.
  • Further development and critical appraisal of methods will enhance understanding and application of these complex molecules.