Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ three-dimensional printing for reparative and regenerative therapy.

Biomedical microdevices·2019
Same author

A Perspective on 3D Bioprinting in Tissue Regeneration.

Bio-design and manufacturing·2019
Same author

Sutureless repair of corneal injuries using naturally derived bioadhesive hydrogels.

Science advances·2019
Same author

Hierarchically Patterned Polydopamine-Containing Membranes for Periodontal Tissue Engineering.

ACS nano·2019
Same author

A Microfabricated Sandwiching Assay for Nanoliter and High-Throughput Biomarker Screening.

Small (Weinheim an der Bergstrasse, Germany)·2019
Same author

A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.

Biofabrication·2019
Same journal

Remodeling TME via feedback-driven photothermal-ferroptosis-immune cascade.

Biomaterials·2026
Same journal

Corrigendum to "Photodynamic therapy produces enhanced efficacy of antitumor immunotherapy by simultaneously inducing intratumoral release of sorafenib" [Biomaterials 2020, 240, 119845].

Biomaterials·2026
Same journal

Mg-integrated octopus-inspired hydrogel dressing enables autonomous adhesion and wound closure for enhanced healing via sequential microenvironment regulation.

Biomaterials·2026
Same journal

Engineering miRNA-223 nanocomplexes via bioorthogonal self-assembly for precision therapy of intervertebral disc degeneration.

Biomaterials·2026
Same journal

Corrigendum to "Enhanced fluorescence imaging guided photodynamic therapy of sinoporphyrin sodium loaded graphene oxide" [Biomaterials 42 (2015) 16442].

Biomaterials·2026
Same journal

An injectable Ce-MnCo LDH nanozyme gel with cascade catalytic activity for acute radiation proctitis in rats.

Biomaterials·2026
See all related articles

Related Experiment Video

Updated: Jun 7, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

A sandwiched microarray platform for benchtop cell-based high throughput screening.

Jinhui Wu1, Ian Wheeldon, Yuqi Guo

  • 1Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.

Biomaterials
|October 23, 2010
PubMed
Summary
This summary is machine-generated.

A novel microarray system enables benchtop screening of chemical libraries for drug discovery. This miniaturized, cell-based assay platform efficiently identifies potential drug candidates from natural compounds and combinatorial libraries.

More Related Videos

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
07:19

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

Related Experiment Videos

Last Updated: Jun 7, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
07:19

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

Area of Science:

  • Biochemistry
  • Drug Discovery
  • Chemical Biology

Background:

  • Expansive compound libraries necessitate efficient screening technologies.
  • High throughput screening (HTS) accessibility remains a challenge in biological research.

Purpose of the Study:

  • To develop a simple, benchtop microarray system for cell-based screening of chemical libraries.
  • To enable miniaturized, reproducible, and scalable screening of individual or combinatorial libraries.

Main Methods:

  • A microarray sandwich system was designed to deliver compounds to isolated cell cultures in microwells.
  • Cell viability was assessed using fluorescence detection after compound exposure.
  • MCF-7 human breast cancer cells were used for toxicology screens.

Main Results:

  • The system successfully identified four hits from toxicology screens.
  • Three hits were found using a combinatorial screen of natural compounds with verapamil (a P-glycoprotein inhibitor).
  • 9-methoxy-camptothecin was identified from screening natural compounds without verapamil.

Conclusions:

  • The developed microarray system offers a robust and portable platform for combinatorial drug screening.
  • This miniaturized HTS approach facilitates the screening of diverse chemical libraries.
  • The system demonstrates potential for broad application in drug discovery and biological research.