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Updated: Jun 7, 2026
08:54
In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
Published on: October 22, 2019
Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death
Takashi Kadono1, Daniel Tran, Rafik Errakhi
1Laboratoire d'Electrophysiologie des Membranes, Université Paris Diderot-Paris 7, Institut de Biologie des Plantes, Bât 630, Orsay, France.
Plos One
|October 23, 2010
Summary
Ozone exposure triggers programmed cell death in plants by activating anion channels. This anion efflux is a crucial early step in the plant
Area of Science:
- Plant biology
- Environmental science
- Cellular signaling
Background:
- Tropospheric ozone is a harmful air pollutant, particularly in urban environments.
- High ozone concentrations can negatively impact plant health and physiology.
Purpose of the Study:
- To investigate the cellular mechanisms underlying ozone-induced plant cell death.
- To identify early molecular events involved in plant responses to ozone stress.
Main Methods:
- Exposure of *Arabidopsis thaliana* suspension cells to ozone.
- Analysis of plasma membrane anion channel activity.
- Investigation of calcium influx and reactive oxygen species (ROS) generation.
- Transcriptional analysis of vacuolar processing enzymes.
Main Results:
- Ozone treatment induced acute cell death in *Arabidopsis thaliana* cells.
- Activation of a plasma membrane anion channel was identified as an early prerequisite for ozone-induced cell death.
- Anion channel activation involves interplay with Ca(2+) influx and NADPH-oxidase generated ROS.
- Ozone exposure promoted the accumulation of transcripts for vacuolar processing enzymes.
Conclusions:
- Anion efflux is an early, key component of ozone-induced programmed cell death.
- Ion channels, particularly anion channels, play a critical role in plant signaling pathways leading to cell death.
- Further research into the roles of ion channels in plant stress responses is warranted.
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