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Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

Mohammad Rashidian1, Jonathan K Dozier, Stepan Lenevich

  • 1Department of Chemistry, University of Minnesota, 207 Pleasant Street, S. E. Minneapolis, MN 55455, USA.

Chemical Communications (Cambridge, England)
|October 23, 2010
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...