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High-throughput SuperSAGE.

Hideo Matsumura1, Kentaro Yoshida, Shujun Luo

  • 1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|October 23, 2010
PubMed
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High-throughput SuperSAGE offers cost-effective digital gene expression profiling by analyzing millions of transcripts. This updated protocol details library multiplexing for enhanced efficiency in gene expression analysis.

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Digital gene expression profiling is crucial for understanding cellular function.
  • Traditional methods can be time-consuming and costly.
  • SuperSAGE provides a high-throughput approach to gene expression analysis.

Purpose of the Study:

  • To present an updated protocol for the High-throughput SuperSAGE method.
  • To highlight the technique of library multiplexing within the SuperSAGE protocol.
  • To enable efficient and cost-effective digital gene expression profiling.

Main Methods:

  • Isolation of 26-bp tag fragments from expressed transcripts using SuperSAGE.
  • Application of ultrahigh-throughput sequencing technologies.

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Last Updated: Jun 7, 2026

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Published on: March 22, 2018

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  • Implementation of library multiplexing for enhanced sample throughput.

    • Main Results:

    • SuperSAGE enables the analysis of millions of transcripts.
    • The method offers reduced cost, effort, and time compared to other techniques.
    • Library multiplexing further optimizes the efficiency of High-throughput SuperSAGE.

    Conclusions:

    • High-throughput SuperSAGE is a powerful tool for digital gene expression profiling.
    • The updated protocol, including library multiplexing, enhances the method's efficiency and cost-effectiveness.
    • This approach facilitates large-scale transcriptomic analysis.