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Updated: Jun 7, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
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Assessing gene-specific methylation using HRM-based analysis.

Ee Ming Wong1, Alexander Dobrovic

  • 1Genetic Epidemiology Laboratory, Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia. ming.wong@petermac.org

Methods in Molecular Biology (Clifton, N.J.)
|October 23, 2010
PubMed
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Methylation-sensitive high-resolution melting (MS-HRM) offers a rapid, in-tube method for DNA methylation analysis. This assay is effective for short DNA fragments from various samples, including biopsies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • DNA methylation analysis is crucial in biomedical research.
  • Current methods are often time-consuming and complex.
  • Need for rapid, efficient DNA methylation analysis techniques exists.

Purpose of the Study:

  • To introduce and evaluate the methylation-sensitive high-resolution melting (MS-HRM) assay.
  • To demonstrate its utility for promoter methylation analysis.
  • To highlight its application in analyzing DNA from challenging sample types.

Main Methods:

  • The MS-HRM assay is an in-tube method.
  • It analyzes sodium bisulphite-modified DNA.
  • It is particularly suited for short DNA fragments.

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Published on: February 24, 2015

Related Experiment Videos

Last Updated: Jun 7, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Published on: September 26, 2015

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Main Results:

  • MS-HRM provides a rapid assessment of DNA methylation.
  • The assay is effective for promoter methylation analysis.
  • It is highly useful for DNA derived from formalin-fixed paraffin-embedded biopsies.

Conclusions:

  • MS-HRM is a valuable tool for rapid DNA methylation analysis.
  • The assay simplifies the process compared to traditional methods.
  • Its efficiency makes it suitable for diverse biomedical and clinical samples.