Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retargeted adenoviruses for local IgA and CD47 blocker production as a novel cancer therapy.

EMBO molecular medicine·2026
Same author

Semi-automated Ribosome Display for High-Throughput DARPin Binder Selection.

New biotechnology·2026
Same author

Transcriptional transactivation turns human iPSC-derived macrophages into an adenovirus-producing cell state.

Journal of virology·2026
Same author

DARPins as pan-reactivators of temperature-sensitive p53 cancer mutants.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Cryo-EM structures of higher order Gephyrin oligomers reveal principles of inhibitory postsynaptic scaffold organization.

Nature communications·2026
Same author

Pre-assembly of biomolecular condensate seeds drives RSV replication.

Nature·2026

Related Experiment Video

Updated: Jun 7, 2026

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Ribosome display: a technology for selecting and evolving proteins from large libraries.

Birgit Dreier1, Andreas Plückthun

  • 1Department of Biochemistry, University of Zürich, Zürich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|October 23, 2010
PubMed
Summary

Ribosome display is a powerful in vitro technology for selecting and improving protein binders. It enables large library diversity and efficient directed evolution for applications in research, diagnostics, and therapy.

More Related Videos

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
10:43

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Related Experiment Videos

Last Updated: Jun 7, 2026

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
10:43

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Protein binders are crucial for biochemical research, diagnostics, and therapeutics.
  • Ribosome display is a potent in vitro selection technology for protein binder development.

Purpose of the Study:

  • To highlight the advantages and applications of ribosome display for protein selection and affinity maturation.

Main Methods:

  • Ribosome display operates entirely in vitro, eliminating the need for cell transformation.
  • Facilitates the handling of large, diverse libraries and the introduction of random mutations via PCR-based methods.
  • Enables iterative cycles of randomization and selection for directed evolution.

Main Results:

  • Ribosome display allows for greater library diversity compared to other techniques.
  • Facilitates convenient introduction of random errors for affinity maturation.
  • Successfully applied for selecting antibody fragments and Designed Ankyrin Repeat Proteins (DARPins).

Conclusions:

  • Ribosome display is a versatile and efficient platform for developing high-affinity protein binders.
  • Its in vitro nature simplifies library handling and enables rapid directed evolution.
  • Significant potential for advancing biochemical research, diagnostics, and therapeutic protein development.