Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 29, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Semi-automated Ribosome Display for High-Throughput DARPin Binder Selection.

Andri Häfliger¹, Sylvie Briand-Schumacher¹, Sven Furler¹

¹Dept. of Biochemistry, University of Zurich, Winterthurerstr. 190, 8057 Zurich, Switzerland.

New Biotechnology

|June 27, 2026

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transcriptional transactivation turns human iPSC-derived macrophages into an adenovirus-producing cell state.

Journal of virology·2026

Same author

DARPins as pan-reactivators of temperature-sensitive p53 cancer mutants.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Cryo-EM structures of higher order Gephyrin oligomers reveal principles of inhibitory postsynaptic scaffold organization.

Nature communications·2026

Same author

Pre-assembly of biomolecular condensate seeds drives RSV replication.

Nature·2026

Same author

Quantitative Degradation Rate Assessment of bioPROTACs Based on Peptide Degrons, E3 Domains, Adapters and Conjugated Small Molecules.

ACS chemical biology·2026

Same author

Genome-replicating HC-AdV: A novel high-capacity adenoviral vector class featuring enhanced <i>in situ</i> payload expression.

Molecular therapy. Methods & clinical development·2025

Same journal

Cell Culture Medium Formulation can be a Driver of Lipoprotein Lipase Release and Polysorbate Degradation Risk in CHO Cell-based Manufacturing Processes.

New biotechnology·2026

Same journal

Advancements in cofactor regeneration for efficient UDP-GlcNAc and UDP-GalNAc synthesis.

New biotechnology·2026

Same journal

Metabolic engineering of Saccharomyces cerevisiae sphingolipid pathways for enhanced phytoceramide production.

New biotechnology·2026

Same journal

Enhancing 3-hydroxypropionic acid production in Komagataella phaffii via methanol and formate co-feeding.

New biotechnology·2026

Same journal

Toward end-to-end continuous biopharmaceutical manufacturing: Current advances and prospects.

New biotechnology·2026

Same journal

Sustainable microbial production of retinol in Yarrowia lipolytica: From strain engineering to formulation.

New biotechnology·2026

See all related articles

High-throughput methods combining Designed Ankyrin Repeat Protein (DARPin) technology with Ribosome Display enable rapid selection and characterization of specific protein binders for diverse applications.

Area of Science:

Biotechnology
Molecular Biology
Protein Engineering

Background:

Designed Ankyrin Repeat Proteins (DARPins) are versatile protein scaffolds.
Ribosome Display is a powerful in vitro selection technology.
Combining DARPins and Ribosome Display yields specific binders for numerous protein targets.

Purpose of the Study:

To detail high-throughput (HT) methods for selecting and characterizing DARPins.
To demonstrate parallel selection capabilities for multiple targets and conditions.
To establish a framework for rapid DARPin discovery and application.

Main Methods:

Development of HT selection strategies for DARPins.
Parallel screening of binders against hundreds of target proteins.

Keywords:

DARPins Designed Ankyrin Repeat Proteins Ribosome Display errorprone, PCR high-throughput screening high-throughput, selections in vitro translation off-rate selection protein engineering protein libraries scaffolds synthetic libraries

More Related Videos

Protein Engineering by Yeast Surface Display

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Related Experiment Videos

Last Updated: Jun 29, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Protein Engineering by Yeast Surface Display

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Downstream characterization of selected DARPin binders.

Structural analysis of DARPin/target complexes.

Main Results:

Successful application of HT methods in numerous DARPin selections for research.
Generation of a wide range of specific DARPin binders.
Demonstration of parallel selection with varied buffers and components.
Summary of DARPin properties, applications, and complex structures.

Conclusions:

The described HT methods provide a robust framework for efficient DARPin discovery.
These methods accelerate the identification and characterization of DARPins for research, diagnostics, and therapy.
The combined DARPin-Ribosome Display approach offers a versatile platform for protein binder generation.