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Updated: Jun 7, 2026

Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

Axoplasm isolation from rat sciatic nerve.

Ida Rishal1, Meir Rozenbaum, Mike Fainzilber

  • 1Department of Biological Chemistry, Weizmann Institute of Science, Israel.

Journal of Visualized Experiments : Jove
|October 26, 2010
PubMed
Summary
This summary is machine-generated.

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This study presents a new protocol for isolating pure axonal cytoplasm (axoplasm) from rat sciatic nerves. This method yields a highly enriched axoplasm preparation, crucial for biochemical research.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Axoplasm isolation is essential for studying axonal functions.
  • Existing methods may lack purity or efficiency.

Purpose of the Study:

  • To develop and describe a reliable protocol for axoplasm isolation from adult rat sciatic nerves.
  • To ensure the purity and enrichment of axonal components in the isolated axoplasm.

Main Methods:

  • Dissection of rat sciatic nerve fascicles and removal of connective tissue.
  • Incubation of nerve segments in hypotonic medium to remove myelin and non-axonal cells.
  • Extraction of the remaining axon-enriched material.

Main Results:

  • A detailed protocol for axoplasm isolation is described.

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Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
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In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
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In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

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Last Updated: Jun 7, 2026

Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
09:09

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

Published on: July 12, 2022

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
08:57

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

Published on: February 10, 2023

  • Proteomic and biochemical analyses confirmed high enrichment of axonal components.
  • The method provides pure axoplasm suitable for further studies.

    • Conclusions:

    • The described protocol offers an effective method for obtaining pure axoplasm.
    • This preparation is valuable for biochemical and proteomic investigations of axonal biology.