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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

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Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

NMR and MS methods for metabonomics.

Frank Dieterle1, Björn Riefke, Götz Schlotterbeck

  • 1Molecular Diagnostics, Novartis Pharma AG, Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|October 26, 2010
PubMed
Summary
This summary is machine-generated.

Metabonomics, or metabolic profiling, systematically analyzes metabolites in biological samples. This guide details best practices for its application in drug development and molecular medicine.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Systems Biology

Background:

  • Metabonomics (metabolomics) profiles metabolites and their temporal changes in biological systems.
  • It offers direct phenotypic insights, contrasting with other '-omics' approaches.
  • Its growing importance is driven by advancements in technology and bioinformatics.

Purpose of the Study:

  • To describe current best practices in metabonomics.
  • To detail essential steps for metabolic profiling in drug development and molecular medicine.

Main Methods:

  • Utilizes advanced analytical techniques like NMR, LC-MS, UPLC-MS, and GC-MS.
  • These platforms enable sensitive and reproducible determination of numerous metabolites.
  • Covers sample preparation, measurement protocols, and data analysis strategies.

Main Results:

  • Provides a comprehensive overview of state-of-the-art metabonomics methodologies.
  • Highlights the critical role of technological and bioinformatics development.
  • Ensures detailed descriptions of all crucial steps in metabolic profiling.

Conclusions:

  • Metabonomics is a powerful tool for understanding biological phenotypes.
  • Adherence to best practices is crucial for reliable results in drug development and molecular medicine.
  • The chapter serves as a detailed guide for researchers in the field.