Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteins that affect DNA conformation.

J J Champoux

    Annual Review of Biochemistry
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Understanding DNA structure and biochemistry is crucial for studying DNA replication, transcription, and recombination. New discoveries about DNA enzymes enhance our knowledge of these fundamental processes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Purification of baculovirus-expressed human DNA topoisomerase I.

    Methods in molecular biology (Clifton, N.J.)·2003
    Same author

    The tyrosyl-DNA phosphodiesterase Tdp1 is a member of the phospholipase D superfamily.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    DNA topoisomerases: structure, function, and mechanism.

    Annual review of biochemistry·2001
    Same author

    Structural alterations in the DNA ahead of the primer terminus during displacement synthesis by reverse transcriptases.

    Journal of molecular biology·2001
    Same author

    Structure-based analysis of the effects of camptothecin on the activities of human topoisomerase I.

    Annals of the New York Academy of Sciences·2001
    Same author

    Assaying DNA topoisomerase I relaxation activity.

    Methods in molecular biology (Clifton, N.J.)·2000
    Same journal

    Lactate as a Chemical Modification on Proteins and Metabolites.

    Annual review of biochemistry·2026
    Same journal

    Nucleocytoplasmic Transport.

    Annual review of biochemistry·2026
    Same journal

    Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.

    Annual review of biochemistry·2026
    Same journal

    Shaping of the Infant Gut Microbiome by Milk Oligosaccharides.

    Annual review of biochemistry·2026
    Same journal

    Proteostasis Deregulation by Metabolism Drives the Hallmarks of Cancer.

    Annual review of biochemistry·2026
    Same journal

    JoAnne Stubbe's Radical Path: A Story of Passion, Curiosity, and Persistence.

    Annual review of biochemistry·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Eukaryotic DNA is organized into chromatin fibers (approx. 100 A diameter) through protein complexation.
    • Higher-order coiling of the unit fiber forms chromatin structure.
    • The precise structure of prokaryotic chromosomes, like in E. coli, remains less defined.

    Purpose of the Study:

    • To highlight the importance of DNA structural features in biochemical studies.
    • To emphasize the impact of recent enzyme discoveries on DNA biochemistry.
    • To underscore the potential for understanding DNA replication, transcription, and recombination.

    Main Methods:

    • Review of recent discoveries in DNA biochemistry.
    • Analysis of the role of DNA enzymes in cellular processes.

    Related Experiment Videos

  • Consideration of DNA structural states in prokaryotes and eukaryotes.

    • Main Results:

    • Discovery of DNA swivelases, DNA gyrase, and DNA unwinding enzymes significantly advances DNA biochemistry.
    • The DNA gyrase suggests a potentially underwound DNA helix state in prokaryotes.
    • Structural features of DNA are critical for biochemical processes.

    Conclusions:

    • Increased knowledge of DNA enzymes and their interactions facilitates understanding of DNA replication, transcription, and recombination.
    • Structural insights into DNA are essential for future biochemical investigations.
    • Further research into DNA enzymes promises detailed insights into DNA metabolism.