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Related Concept Videos

Toxoplasmosis01:28

Toxoplasmosis

Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 7, 2026

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
09:52

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

A novel multifunctional oligonucleotide microarray for Toxoplasma gondii.

Amit Bahl1, Paul H Davis, Michael Behnke

  • 1Genomics and Computational Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.

BMC Genomics
|October 27, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed a cost-effective microarray for pathogen research, enabling comprehensive transcriptome analysis and SNP genotyping for Toxoplasma gondii and Plasmodium falciparum. This tool provides insights into parasite gene expression and genetic variation.

Related Experiment Videos

Last Updated: Jun 7, 2026

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
09:52

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

Area of Science:

  • Parasitology
  • Genomics
  • Molecular Biology

Background:

  • Microarrays are essential for genome analysis but are often expensive.
  • Developing cost-effective methods for pathogen research is crucial.
  • Reducing probe numbers while maintaining functionality is a key challenge.

Purpose of the Study:

  • To design a single, low-cost oligonucleotide microarray for broad applications in pathogen research.
  • To enable genome-wide expression profiling and single-nucleotide polymorphism (SNP) genotyping for Toxoplasma gondii and Plasmodium falciparum.
  • To provide a comprehensive view of the T. gondii transcriptome and resolve recombination points.

Main Methods:

  • Utilized available genome sequences and annotations for T. gondii and P. falciparum.
  • Designed a multifunctional oligonucleotide microarray with a reduced probe set.
  • Performed genome-wide expression profiling and SNP-based genotyping.
  • Analyzed T. gondii transcriptome across major lineages and identified new SNPs.

Main Results:

  • Developed a cost-effective microarray supporting diverse applications for T. gondii and P. falciparum.
  • Generated a comprehensive view of the T. gondii tachyzoite transcriptome, with ~49% of genes expressed and 26% differentially expressed among strains.
  • Achieved high-confidence genotyping using a novel probe design, enabling resolution of recombination points.
  • Identified over 620,000 new SNPs in T. gondii, with ~11,000 intersecting expression probes.

Conclusions:

  • The multifunctional microarray provides a global view of the T. gondii transcriptome and detailed recombination analysis.
  • The array's design strategy enhances analyses by allowing probes to be used for unintended purposes.
  • This cost-effective microarray is widely adopted by the T. gondii research community and its design is applicable to other pathogens.