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Related Experiment Video

Updated: Jun 7, 2026

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

E2F-1 directly regulates thrombospondin 1 expression.

Wei Ji¹, Wei Zhang, Wuhan Xiao

¹Key Laboratory of Biodiversity and Conservation of Aquatic Organisms, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China.

|October 27, 2010

Summary

E2F-1 transcription factor activates Thrombospondin 1 (TSP1) gene expression. This finding reveals E2F-1 as a regulator of TSP1, impacting angiogenesis and potentially tumor growth.

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Area of Science:

Molecular Biology
Cancer Research
Cell Biology

Background:

Thrombospondin 1 (TSP1) inhibits angiogenesis, suppressing tumor growth and metastasis.
Understanding TSP1 regulators is crucial for elucidating its biological functions.

Purpose of the Study:

To investigate the regulatory mechanisms controlling TSP1 gene expression.
To identify upstream regulators of TSP1 and their role in angiogenesis.

Main Methods:

Promoter assays and Northern blot analysis to assess TSP1 transcription.
Site-directed mutagenesis of the TSP1 promoter to identify regulatory regions.
Quantitative real-time PCR and chromatin immunoprecipitation (ChIP) assays.
Western blot analysis to study the effect of pRB1.

Main Results:

E2F-1 activates TSP1 transcription via a specific responsive element (-144/-137) in the TSP1 promoter.
Knockdown of E2F-1 reduces TSP1 promoter activity, confirming in vivo relevance.
E2F-1 directly binds to the identified promoter region.
pRB1 dose-dependently suppresses E2F-1-induced TSP1 activation.

Conclusions:

TSP1 is a novel transcriptional target of E2F-1.
E2F-1 regulates angiogenesis by modulating TSP1 expression.