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Updated: Jun 7, 2026

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Published on: October 25, 2019
Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring
M Carmen Galan1, Anh Tuan Tran, Claire Bernard
1School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK. M.C.Galan@bristol.ac.uk
Abstract:
A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.
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