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Related Concept Videos

ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
Stomach pH Regulation01:21

Stomach pH Regulation

The human body carefully regulates the internal pH of different organs to maintain homeostasis. For example, while the blood plasma maintains a neutral pH of 7, the stomach lumen has an acidic pH of 1.5 - 3.5. The low pH of stomach lumen helps kill pathogens in the food and break down complex food molecules.
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
Renal Regulation of Acid-Base Balance01:29

Renal Regulation of Acid-Base Balance

Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
Acid Suppressive Drugs for Peptic Ulcer Disease: Proton Pump Inhibitors01:13

Acid Suppressive Drugs for Peptic Ulcer Disease: Proton Pump Inhibitors

Peptic ulcers, often induced by H. pylori infections or NSAID usage, arise from disruptions in the delicate balance of gastric acid production. Peptic ulcers stem from heightened gastric acid levels due to H. pylori infections or NSAID use. The protective mucus layer diminishes in the presence of these factors, allowing gastric acid to erode the stomach lining and form ulcers.
Gastric acid, a potent cocktail of hydrogen and chloride ions, is produced in specialized parietal cells within the...

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Related Experiment Video

Updated: Jun 7, 2026

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
07:38

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015

Pancreatic bicarbonate secretion involves two proton pumps.

Ivana Novak1, Jing Wang, Katrine L Henriksen

  • 1Department of Biology, August Krogh Building, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen, Denmark. inovak@bio.ku.dk

The Journal of Biological Chemistry
|October 28, 2010
PubMed
Summary
This summary is machine-generated.

Pancreatic ducts secrete bicarbonate, crucial for digestion. New research reveals that gastric and non-gastric H(+)-K(+) pumps actively contribute to this secretion, revising our understanding of pancreatic bicarbonate transport.

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Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
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10:39

Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

Published on: July 5, 2013

Area of Science:

  • Gastroenterology
  • Cell Physiology
  • Biochemistry

Background:

  • Pancreatic secretion contains bicarbonate, vital for neutralizing duodenal acid and activating digestive enzymes.
  • Existing models of pancreatic bicarbonate secretion, primarily involving H(+)/HCO(3)(-) transporters and Na(+)/K(+)-ATPase, do not fully explain high bicarbonate concentrations.
  • The role of other active transporters, specifically pumps, in pancreatic secretion has remained largely unexplored.

Purpose of the Study:

  • To investigate the presence and function of H(+)-K(+)-ATPases in pancreatic ducts.
  • To determine the contribution of these pumps to pancreatic fluid and bicarbonate secretion.
  • To re-evaluate the physiological model of bicarbonate transport in the pancreas.

Main Methods:

  • Isolated rat pancreatic ducts were used to measure intracellular pH and secretion rates.
  • The effects of H(+)-K(+) pump inhibitors and ion substitutions were assessed.
  • RNA and protein expression of gastric and non-gastric H(+)-K(+) pumps were analyzed, and their localization determined.

Main Results:

  • Pancreatic ducts were found to express functional gastric and non-gastric H(+)-K(+)-ATPases.
  • These pumps are located in the plasma membranes of pancreatic duct epithelial cells.
  • Quantitative analysis indicates that H(+)-K(+) pumps significantly contribute to pancreatic secretion across multiple species.

Conclusions:

  • The discovery of functional H(+)-K(+) pumps in pancreatic ducts necessitates a revision of current bicarbonate transport models.
  • These findings highlight a previously unrecognized mechanism contributing to pancreatic secretion.
  • Understanding the role of H(+)-K(+) pumps is crucial for elucidating the pathophysiology of pancreatic diseases.