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Updated: Jun 7, 2026
07:07
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Published on: July 19, 2019
A digest of protein purification
Dermot Walls1, Robert McGrath, Sinéad T Loughran
1School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland. Dermot.walls@dcu.ie
Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2010
Summary
This review summarizes protein chromatography advancements, detailing methods that exploit protein properties for isolation. It also provides resources to aid researchers in protein purification processes.
Area of Science:
- Biochemistry
- Proteomics
- Analytical Chemistry
Background:
- Protein purification is essential for biochemical and proteomic research.
- Classical chromatography relies on protein characteristics like size and binding affinity.
- Molecular biology and bioinformatics have advanced protein purification techniques.
Purpose of the Study:
- To review developments in protein chromatography.
- To compile resources for protein purification.
Main Methods:
- Review of classical and modern chromatographic techniques.
- Discussion of advances driven by molecular biology and bioinformatics.
- Compilation of available tools and resources.
Main Results:
- Significant progress in protein chromatography has been achieved.
- High-throughput proteomic platforms have emerged.
- A range of resources are available to support purification.
Conclusions:
- Protein chromatography continues to evolve with technological advancements.
- Integrated approaches enhance the efficiency of protein isolation.
- Resources exist to assist researchers in optimizing purification strategies.
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