Related Experiment Video
Updated: Jun 7, 2026
07:39
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
Published on: June 25, 2018
Literature-based discovery of diabetes- and ROS-related targets.
Junguk Hur1, Kelli A Sullivan, Adam D Schuyler
1Bioinformatics Program, University of Michigan, Ann Arbor, MI 48109, USA.
BMC Medical Genomics
|October 29, 2010
Summary
This study identifies genes linked to reactive oxygen species (ROS) and diabetes using text mining. Key ROS-diabetes genes were found to be differentially expressed in mouse models of diabetic neuropathy.
Area of Science:
- Biomedical Informatics
- Molecular Biology
- Genetics
Background:
- Reactive oxygen species (ROS) are implicated in cellular damage in diseases like diabetes.
- A comprehensive database for ROS-associated genes is lacking.
- Understanding these genes is crucial for addressing diabetic complications.
Purpose of the Study:
- To identify and compile genes related to both ROS and diabetes.
- To evaluate the biological relevance of these identified genes in diabetic neuropathy.
- To establish a foundation for a ROS-diabetes gene database.
Main Methods:
- Utilized text mining technology (SciMiner) on 1,154 PubMed-indexed papers on ROS and diabetes.
- Identified 1,026 ROS- and diabetes-related targets.
- Compared target frequency against random literature to find over-represented genes.
- Measured expression levels of nine selected genes in mouse dorsal root ganglia (DRG).
Main Results:
- Identified 1,026 ROS- and diabetes-related targets.
- Found 53 significantly over-represented targets, including catalase, NADPH oxidase, and superoxide dismutase families.
- Eight of nine tested genes showed differential expression in diabetic mice.
- Six genes' expression changes correlated with increased oxidative stress in the DRG.
Conclusions:
- Literature mining effectively compiled ROS-diabetes related targets.
- Selected targets demonstrated biological relevance in the pathogenesis of diabetic neuropathy.
- This work provides a basis for further research into ROS-mediated diabetic complications.
Related Concept Videos
01:29Pharmacogenomics: Identification of New Drug Targets
Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
01:27Diabetic Retinopathy
DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...
01:24Type II Diabetes II: Pathophysiology
PathophysiologyType 2 diabetes mellitus (T2DM ) is a chronic metabolic disorder characterized by insulin resistance and progressive pancreatic β-cell dysfunction, leading to impaired glucose homeostasis. It results from interactions among genetic predisposition, environmental factors, and metabolic stressors, such as overnutrition and a sedentary lifestyle.Insulin Resistance and Glucose DysregulationEarly T2DM involves insulin resistance in skeletal muscle, adipose tissue, and the liver.
01:26Type I Diabetes II: Pathophysiology
Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular uptake of...
01:25Type II Diabetes Mellitus III: Clinical Manifestations and Diagnosis
Type 2 diabetes mellitus develops gradually and is often asymptomatic in early stages.Clinical ManifestationsWhen symptoms appear, they include fatigue, blurred vision, pruritus, delayed wound healing, and recurrent infections, particularly candidal infections. Peripheral neuropathy may present as numbness or tingling in the extremities. Classic hyperglycemia symptoms—polyuria, polydipsia, and polyphagia—are less common. Most patients are overweight and frequently have associated hypertension...
01:20Pathophysiology of Diabetes
Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility, suggesting a...
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility, suggesting a...