Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

The Journal of cell biology·2026
Same author

Transcription-coupled nucleotide excision repair protects against genomic instability and cell death induced by the liver toxin methyleugenol.

Cell death & disease·2026
Same author

Crosstalk between chromatin state and ATM signalling in DNA damage-induced transcription stress.

The EMBO journal·2025
Same author

Corrigendum to 'A randomised phase 2 immunogenicity and safety study of a MF59-adjuvanted quadrivalent subunit inactivated cell-derived influenza vaccine (aQIVc) in adults aged 50 years and older' Vaccine 51 (2025) 126791.

Vaccine·2025
Same author

Transcription-coupled repair: tangled up in convoluted repair.

The FEBS journal·2025
Same author

Editorial.

DNA repair·2025
Same journal

Evolutionary and Biochemical Perspectives on the Incorporation and Utilization of Selenocysteine.

Cold Spring Harbor perspectives in biology·2026
Same journal

The Mitochondrial Calcium Uniporter: From Parts to Signaling Networks.

Cold Spring Harbor perspectives in biology·2026
Same journal

Growth Control and Beyond: Functional Diversity and Regulation of the Hippo Pathway in the Nervous System.

Cold Spring Harbor perspectives in biology·2026
Same journal

Structural Studies of Core Hippo Pathway Components.

Cold Spring Harbor perspectives in biology·2026
Same journal

The Hippo Pathway in Intestinal Regeneration, Fetal Reprogramming, and Tumorigenesis.

Cold Spring Harbor perspectives in biology·2026
Same journal

A Synergy between Genetics and Biochemistry Unravels the Molecular Architecture of the Hippo Signaling Pathway.

Cold Spring Harbor perspectives in biology·2026
See all related articles

Related Experiment Video

Updated: Jun 7, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

DNA damage response.

Giuseppina Giglia-Mari1, Angelika Zotter, Wim Vermeulen

  • 1Department of Genetics, Erasmus University Medical Center, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.

Cold Spring Harbor Perspectives in Biology
|October 29, 2010
PubMed
Summary
This summary is machine-generated.

DNA damage disrupts essential cell functions and contributes to aging and cancer. A complex DNA damage response (DDR) network, involving repair pathways and checkpoints, maintains genomic stability through precise spatial and temporal control.

More Related Videos

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Experiment Videos

Last Updated: Jun 7, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Structural DNA changes impair vital cellular processes like replication and transcription.
  • These alterations are implicated in age-related diseases and cancer development.
  • Genomic integrity is protected by a complex network of DNA damage response (DDR) mechanisms.

Purpose of the Study:

  • To elucidate the spatio-temporal orchestration of the DNA damage response (DDR).
  • To understand how DDR mechanisms safeguard genomic integrity.
  • To explore the role of DDR in preventing age-related diseases and cancer.

Main Methods:

  • Review of existing literature on DNA repair pathways.
  • Analysis of chromatin dynamics during DNA damage response.
  • Investigation of cell-cycle checkpoint regulation.

Main Results:

  • DNA damage response (DDR) is a chromatin-associated process.
  • DDR requires precise temporal and spatial control.
  • Multiple repair pathways, tolerance processes, and checkpoints form the DDR network.

Conclusions:

  • The intricate network of DNA damage response (DDR) mechanisms is crucial for maintaining genomic stability.
  • Effective spatio-temporal orchestration of DDR is essential to counteract DNA damage.
  • Understanding DDR is key to addressing age-related diseases and cancer.