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Updated: Jun 7, 2026
Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Improved selective and differential medium for isolation of Escherichia coli O157:H7
Sang-Hyun Park1, Sangryeol Ryu, Dong-Hyun Kang
1Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
Abstract:
GMAC, a modified version of Sorbitol MacConkey medium (SMAC), was produced with a reduced quantity of selective agents and incorporated gentiobiose. GMAC supported a higher recovery rate of heat- or acid-injured Escherichia coli O157:H7 cells than SMAC with cefixime and tellurite (CT-SMAC), while differentiating E. coli O157:H7 from sorbitol-nonfermenting Hafnia alvei.
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