Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates
Paul P Geurink1, Bogdan I Florea, Gijs A Van der Marel
1Leiden Institute of Chemistry and Netherlands Proteomics Centre, Gorlaeus Laboratories, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
Chemical Communications (Cambridge, England)
|October 29, 2010
Abstract:
Tri-functional activity-based protein probes that encompass an electrophilic trap, a photo-reactive group and a bio-orthogonal ligation handle are described. With these, and in a three-step chemical proteomics approach, proteasomal catalytic sites are covalently and irreversibly modified, followed by photocrosslinking of these to flanking subunits and Staudinger-Bertozzi ligation for visualization and identification of the resulting conjugates.
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