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Updated: Jun 7, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Molecular mass spectrometry imaging in biomedical and life science research
Jaroslav Pól1, Martin Strohalm, Vladimír Havlíček
1Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Academy of the ASCR, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Abstract:
This review describes the current state of mass spectrometry imaging (MSI) in life sciences. A brief overview of mass spectrometry principles is presented followed by a thorough introduction to the MSI workflows, principles and areas of application. Three major desorption-ionization techniques used in MSI, namely, secondary ion mass spectrometry (SIMS), matrix-assisted laser desorption ionization (MALDI), and desorption electrospray ionization (DESI) are described, and biomedical and life science imaging applications of each ionization technique are reviewed. A separate section is devoted to data handling and current challenges and future perspectives are briefly discussed at the end.
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