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Related Experiment Videos

[Urinary iodide determination by X-ray fluorescence].

A Turzo1, J M Legendre, R Quiniou

  • 1Service de Médecine Nucléaire et de Biophysique, CHU A., Brest.

Annales De Biologie Clinique
|January 1, 1990
PubMed
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X-ray fluorescence accurately measures urinary iodide, directly or after resin concentration. This method offers a simple, reliable approach for determining iodide levels in various concentrations.

Area of Science:

  • Analytical Chemistry
  • Nuclear Chemistry

Background:

  • Urinary iodide levels are crucial indicators of thyroid health and iodine status.
  • Accurate and simple measurement methods are needed for clinical and research applications.

Purpose of the Study:

  • To evaluate X-ray fluorescence (XRF) for quantifying urinary iodide.
  • To establish a reliable method for both high and normal urinary iodide concentrations.

Main Methods:

  • Direct XRF measurement of urinary solution.
  • Anionic resin concentration followed by XRF analysis for low iodide levels.
  • Utilized a (241)Am radioactive source for excitation.

Main Results:

  • Established proportionality between X-ray counts and iodide mass up to 400 micrograms.

Related Experiment Videos

  • Achieved detection limits of 0.44 microgram (solid matrix) and 0.9 microgram/ml (solution).
  • Normal urinary iodide range in healthy subjects found to be 53 ± 22 micrograms/l.

    • Conclusions:

    • X-ray fluorescence provides a simple and accurate method for urinary iodide determination.
    • The method is applicable to both high and normal iodide concentrations, with resin concentration necessary for the latter.
    • XRF offers a reliable alternative to existing methods, distinguishing iodide from total iodine in specific cases.