Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multispot, multianalyte, immunoassay.

R Ekins1, F Chu, E Biggart

  • 1Department of Molecular Endocrinology, University College, Middlesex School of Medicine, London, UK.

Annales De Biologie Clinique
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visual Event-Related Potentials under External Emotional Stimuli as Early Signs for Mild Cognitive Impairment.

The journal of prevention of Alzheimer's disease·2024
Same author

Detection of significant liver fibrosis in Chinese psoriasis patients receiving methotrexate: a comparison between transient elastography and liver histology.

Hong Kong medical journal = Xianggang yi xue za zhi·2024
Same author

[Study of the predictive role of serum HBV RNA on HBeAg serological conversion in children with chronic hepatitis B].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2024
Same author

Multi-camera imaging to characterize jet and liner uniformity on the Plasma Liner Experiment (PLX).

The Review of scientific instruments·2023
Same author

Experimental Measurements of Ion Diffusion Coefficients and Heating in a Multi-Ion-Species Plasma Shock.

Physical review letters·2023
Same author

Step-by-step illustrated guide to central neck dissection.

The Journal of laryngology and otology·2021
Same journal

A morphology-driven proof-of-concept study linking interphase nuclear abnormalities to dicentric-mediated genomic instability.

Annales de biologie clinique·2026
Same journal

Analytical validation and clinical concordance of Diazyme free light chain assays on the Cobas® platform compared with Freelite™.

Annales de biologie clinique·2026
Same journal

Small-cell secondary plasma cell leukemia mimicking low-grade B-cell lymphoma.

Annales de biologie clinique·2026
Same journal

[Multiple myeloma and analytical discrepancy in serum free light chain assay: a case report].

Annales de biologie clinique·2026
Same journal

[Summary of the 34th National Conference of the National College of Hospital Biochemistry].

Annales de biologie clinique·2026
Same journal

Assessment of the carbon footprint of an automated biochemistry toxicology platform: feedback from Rouen University Hospital.

Annales de biologie clinique·2026
See all related articles

Highly sensitive immunoassays can be developed using minimal sensor antibody. This enables microspot immunoassay arrays for measuring numerous substances in small samples, like a drop of blood.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Immunology

Background:

  • Conventional immunoassays often require substantial antibody quantities.
  • Optimizing immunoassay sensitivity and efficiency is crucial for diagnostics.

Purpose of the Study:

  • To explore the development of highly sensitive immunoassays using reduced sensor antibody amounts.
  • To introduce microspot immunoassay arrays for multiplexed analyte detection.

Main Methods:

  • Theoretical consideration of immunoassay design principles.
  • Development of ratiometric immunoassays using labelled sensor and developing antibodies.
  • Implementation of microspot technology with fluorescent labels and laser scanning.

Main Results:

Related Experiment Videos

  • Demonstrated that fractional occupancy is independent of sample volume and antibody concentration with reduced antibody amounts.
  • Achieved microspot immunoassay sensitivity comparable to conventional macroscopic assays.
  • Proposed immunoassay arrays capable of measuring multiple analytes in minute sample volumes.

Conclusions:

  • Minimal sensor antibody amounts can yield highly sensitive immunoassays.
  • Microspot technology and ratiometric detection enable sensitive, multiplexed immunoassay arrays.
  • This approach facilitates the analysis of numerous substances in small biological samples.