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Conditions for the extraction of protease-free LMG proteins from calf thymus
J P Durand1, F Goudard, J Pieri
1Laboratoire de Biochimie et Radiobiochimie, Faculté des Sciences, Université de Nantes, France.
Biochemistry International
|December 1, 1990
Abstract:
Calf thymus LMG proteins are known to be susceptible to a wide variety of chromatin-associated proteases. We have investigated the use of different inhibitors to protect LMG chromosomal proteins from proteolytic degradation. In this work we have developed a preparative method for the extraction of LMG proteins from purified chromatin which provides an LMG protein extract devoid of proteolytic activity.