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A monoclonal antibody recognizing selectively rat granulocytes.
1Department of Immunology, University of Göttingen, Germany.
Immunobiology
|November 1, 1990
Summary
A new mouse antibody, RK-4, specifically targets rat granulocytes and their precursors. This antibody is a valuable tool for researching granulocyte differentiation and function in various conditions.
Area of Science:
- Immunology
- Hematology
Background:
- Granulocytes play crucial roles in immune responses.
- Specific markers for granulocyte identification are essential for research.
Purpose of the Study:
- To describe a novel murine monoclonal antibody, RK-4 (IgG1).
- To characterize the specificity and utility of RK-4 for rat granulocyte research.
Main Methods:
- Murine monoclonal antibody production and characterization.
- Immunocytological analysis.
- Flow cytometry.
- Cryosection analysis of various organs.
Main Results:
- RK-4 (IgG1) specifically recognizes a surface antigen on rat neutrophils, eosinophils, and basophils.
- No cross-reactivity was observed with other cellular structures in lymphatic or non-lymphatic organs.
- Flow cytometry confirmed granulocytes constitute 15-20% of peripheral blood leukocytes.
Conclusions:
- RK-4 is a highly specific antibody for rat granulocytes and their precursors.
- This antibody serves as a valuable tool for investigating granulocyte biology.