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Diode array detection of low level co-eluting species in high-performance liquid chromatography
N H Anderson1, M R Gray, C J Hinds
1Sterling Research Group, Alnwick, Northumberland, UK.
Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1990
Abstract:
A simple and sensitive method for detecting low levels (0.5 and 1.0%, w/w) of co-eluting species in HPLC has been developed. This method is based on the subtraction of normalized peak up-slope and down-slope spectra from that of the apex. Visual inspection of the resultant "difference spectra" allows for a qualitative judgement regarding the integrity of the peak under consideration.