Related Experiment Videos
Effect of mannozym treatment on plasma fibronectin concentration in germfree and conventional mice
1Institute of Microbiology, Third Department of Medicine, Semmelweis University Medical School, Budapest, Hungary.
Abstract:
As a result of a single intraperitoneal treatment with 1 mg/ml of Mannozym (M) the plasma fibronectin (FN) level was significantly increased both in germfree (Gf) and conventional (Cv) mice, and though it started from a lower value in Gf mice, it rose to a similar level to that of Cv animals. The maximum of FN level was observed on the first day after treatment both in Gf and Cv mice. It had returned to normal by the 7th day in Cv mice, but it was higher in Gf mice as compared to untreated controls even on the 14th day. Thus the increase of FN level was of higher degree and longer duration following M treatment in Gf mice than in Cv animals. In treated Gf mice the plasma FN concentration was in the same range as in untreated Cv mice even at the termination of experiment. Both in Gf and Cv mice, there was a relative spleen weight increase, the degree of which was similar, but the duration was longer in Gf mice than in Cv ones.
Insights
Mannozym treatment significantly boosts plasma fibronectin (FN) levels in mice. Germfree mice showed a more pronounced and prolonged FN increase compared to conventional mice.
Area of Science:
- Immunology
- Microbiology
- Biochemistry
Background:
- Plasma fibronectin (FN) plays a crucial role in immune responses and tissue repair.
- Germfree (Gf) and conventional (Cv) mice exhibit distinct physiological differences.
- Understanding the impact of exogenous substances on FN levels is important for immune modulation research.
Purpose of the Study:
- To investigate the effect of Mannozym (M) on plasma fibronectin levels in germfree and conventional mice.
- To compare the duration and magnitude of fibronectin response between Gf and Cv mice after Mannozym administration.
Main Methods:
- Intraperitoneal administration of Mannozym (1 mg/ml) to Gf and Cv mice.
- Measurement of plasma fibronectin levels at various time points post-treatment.
- Assessment of relative spleen weight changes.
Main Results:
- Mannozym significantly increased plasma fibronectin levels in both Gf and Cv mice.
- The increase in fibronectin was more pronounced and sustained in Gf mice compared to Cv mice.
- A relative spleen weight increase was observed in both groups, with a longer duration in Gf mice.
Conclusions:
- Mannozym effectively modulates plasma fibronectin levels.
- Germfree mice exhibit a heightened and prolonged fibronectin response to Mannozym compared to conventional mice.
- These findings suggest potential applications of Mannozym in immune system modulation, particularly in contexts where enhanced fibronectin activity is desired.