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Oligonucleotides with pyrene fluorophore at the sugar fragment: synthesis and properties in binding to complementary
1Department of Applied Chemistry, Himeji Institute of Technology, Japan.
Nucleic Acids Symposium Series
|January 1, 1990
Abstract:
Oligonucleotides with 1-pyrenylmethyl substituent at the designated sugar residue were synthesized by using 5'-dimethoxytrityl 2'-(1-pyrenylmethyl)uridine 3'-phosphorobisdiethylamidite 1. It was shown that the pyrene-oligonucleotides have enhanced affinity in binding to the complementary polynucleotide sequence. The fluorescence yield and life time of the pyrene-oligonucleotide was drastically enhanced when bound by the complementary polynucleotide.