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Recognition of tRNA identity determinants by aminoacyl-tRNA synthetases
T Muramatsu1, O Nureki, H Kanno
1Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Nucleic Acids Symposium Series
|January 1, 1990
Abstract:
By analyzing aminoacylation activities of variants of isoleucine tRNAs and glutamic acid tRNA from Escherichia coli, it was found that the anticodons are the major determinants for "identities" of these tRNAs. It was also shown that the post-transcriptional modifications are essential to aminoacylation of these tRNAs. Interactions between the tRNAs and the aminoacyl-tRNA synthetases were analyzed by NMR spectroscopy.