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Purity determination by a combination of spectrophotometric and liquid chromatographic methods: application to
1Bristol-Myers Squibb, Pharmaceutical Research Institute, Buffalo, NY 14213.
Journal of Chromatographic Science
|October 1, 1990
Abstract:
A method is described to determine the purity of 13-cis-retinoic acid. Spectrophotometry is used to determine the non-UV-absorbing impurities, and HPLC is used to determine the UV-absorbing impurities. In combination, these methods permit determination of the purity. An authentic standard is used in developing this method, but it is not needed in the application of the method.