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Updated: Jun 7, 2026

Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Published on: July 22, 2016
[Transformation of human bronchial epithelial cells BEP2D induced by ²³⁸Pu α-particles]
1Institute of Radiation Medicine, Academy of Military Medicine Science , Beijing 100850, P . R. China.
Background:
To establish a model for the study of transformation of human cell by radon and its progeny.
Methods:
BEP2D, a HPV18 immortalized human bronchial epithelial cell line, was irradiated with high-energy α-particles emitted by ²³⁸Pu.
Results:
A single 1. 5 Gy dose of α-particles induced transformation of the cells. A series of sequential steps arose among transformed cells, including altered growth kinetics, resistance to serum-induced terminal differentiation, and anchorage-independence growth.
Conclusions:
This cell line may provide a model to study the cellular and molecular changes at various stages in radiation carcinogenesis.
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