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[Cell cycle arrest and apoptosis induced by arsenic trioxide in human lung cancer cell line]
1Department of Center Laboratory, Union Hospital, Tongji Medical University, Wuhan, Hubei 430022, P . R. China.
Background:
To explore the potential therapeutic effect of arsenic trioxide(As₂O₃ ) on human lung cancer.
Methods:
Cell growth curves, cell proliferation, cell cycle and apoptosis of human lung cancer cell line GLC-82 were detected by theMTT method and flowcytometry (FCM) .
Results:
The data showed that As₂O₃ significantly inhibited proliferation of GLC-82 cells and the inhibiting effects had a dose-and time-dependence. The cell proliferation inhibition rate was 81. 05% when treated with 4. 0 Lmol/L As₂O₃ for 96 hours. The change of DNA content of GLC-82 cells indicated that As₂O₃ was able to block cell cycle progress in G2/M phase, with appearance of sub-G1 peak in a dose-dependent manner.
Conclusions:
As₂O₃ can effectively inhibit the proliferation of human lung cancer cell line GLC-82 and the possible mechanisms may be related to G2/M phase arrest and apoptosis induced by As₂O₃.
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